4 103 Neural and affective responses to prolonged eye contact with parents and unfamiliar others in depressed and non-depressed adolescents SUPPLEMENTARY MATERIALS SUPPLEMENT S4.1 Study procedure Families with an HC adolescent were recruited via public advertisements and (online) social media, including Facebook and advertisement in the monthly magazine of the Royal Dutch Touring Club (ANWB). Families with a depressed adolescent were recruited via mental health facilities. Parents and adolescents were briefed about the study and underwent a comprehensive telephone screening during which family circumstances and informed consent were discussed and adolescents were pre-screened for (a history of) psychiatric disorders. Families were invited for two appointments: An assessment day in the lab and an MRI session on a separate day. Prior to the first appointment adolescents were asked to fill out an online questionnaire battery including demographics and clinical and cognitive measures (see Supplement S4.5 for details). During the first appointment, families performed parent-adolescent interaction tasks and filled out additional questionnaires. During the second appointment, adolescents underwent an MRI scan at the LUMC in Leiden, the Netherlands. Prior to and after the scan, adolescents filled out a set of questionnaires, received instructions about the MRI tasks, and performed some practice trials. Adolescents performed four tasks in the MRI scanner: The eye contact task as described below, the parental social feedback task (van Houtum et al., 2022), a peer evaluation task (van Houtum, wever, et al., 2023), and an autobiographical memory task (van Houtum, van Schie, et al., 2023). Upon completion of the MRI scans, adolescents were fully debriefed about the goals of the study and received a monetary compensation and travel allowance. All participants provided written informed consent for each individual testing day. The median of days between the first and second appointment was 42 days (range: 7-265 days) and did not significantly differ between depressed and non-depressed adolescents (t(76) = -0.77, p = .444).
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