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4 91 Neural and affective responses to prolonged eye contact with parents and unfamiliar others in depressed and non-depressed adolescents Figure 4.1 Displays and timings of video stimuli of an unfamiliar adult with a direct and averted gaze in the eye contact task. Videos of adolescents’ parent were contrasted with videos of an unfamiliar same-sex parent, an unfamiliar peer (same-sex adolescent), and videos of the self. Data preprocessing and analyses Affective and gaze responses Adolescents’ affective and gaze responses were analyzed in R (R Core Team (2013), version 3.6.1) with the following packages: Lme4 for mixed model analyses, psych for descriptive statistics, and ggplot2 for data visualization (Bates et al., 2012; Revelle, 2012; Wickham et al., 2016). Questions that were not answered and/or confirmed were excluded from all affective response analyses, which resulted in 0.5% missing responses (11/ 2360 trials) in HC adolescents and 0.3% (2/760 trials) in DEP adolescents. Eye tracking analyses Eye movements were recorded with a tower mounted monocular EyeLink 1000Hz MRIcompatible eye tracker (SR Research Ltd., Mississauga, Ontario, Canada), placed inside the

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